myhc iib (Developmental Studies Hybridoma Bank)
Structured Review

Myhc Iib, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 808 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/myhc+iib/anti-Myosin+heavy+chain+Type+IIB/pmc13170884-92-18-24
Average 96 stars, based on 808 article reviews
Images
1) Product Images from "Alaska pollock protein as a functional dietary source for promoting skeletal muscle hypertrophy and lipid metabolic remodeling"
Article Title: Alaska pollock protein as a functional dietary source for promoting skeletal muscle hypertrophy and lipid metabolic remodeling
Journal: PLOS One
doi: 10.1371/journal.pone.0348366
Figure Legend Snippet: Immunofluorescence staining of the superficial region of the gastrocnemius muscle at (a) 90 and (b) 210 min post-feeding. Black indicates MyHC IIx–positive fibers, and green indicates MyHC IIb–positive fibers. The cross-sectional area of each fiber was calculated, and data are presented as the mean ± SEM. Statistical comparisons were performed using Student’s t -test (90 min: N = 3; 210 min: N = 4). *; p < 0.05, **; p < 0.01. Scale bar = 100 μm.
Techniques Used: Immunofluorescence, Staining
Related Articles
Incubation:Article Title: Displaced myonuclei are attributable to both resident myonuclear migration and stem cell fusion during mechanical loading in adult skeletal muscle Article Snippet: .. For fibre type assessments, sections were incubated in primaries for Article Title: Alaska pollock protein as a functional dietary source for promoting skeletal muscle hypertrophy and lipid metabolic remodeling Article Snippet: .. Sections were fixed with acetone and incubated overnight at 4°C with primary antibodies against MyHC IIa (SC-71) and Article Title: Displaced myonuclei are attributable to both resident myonuclear migration and stem cell fusion during mechanical loading in adult skeletal muscle. Article Snippet: .. For fibre type assessments, sections were incubated in primaries for Article Title: Atrazine exposure induces skeletal muscle atrophy: Multi-omics insights into mechanisms and therapeutic targets. Article Snippet: .. Frozen TA muscle sections were fixed in 4 % paraformaldehyde (PFA), permeabilized/blocked in PBST containing 0.2 % Triton X‐100 and 10 % normal goat serum at room temperature (RT) for 30 min, then incubated with 3 % affinipure Fab fragment anti-mouse IgG(H+L) and anti-mouse IgM (Jackson Immuno Research, West Grove, PA, catalog 115–007–003 and 115–006–020) at RT for 1 h. Primary antibodies used included MyHC-IIA (1:40, DSHB, SC-71), Article Title: Non-muscle myosin IIC predominantly expressed in the slow-twitch skeletal muscles impedes age-related muscle weakness. Article Snippet: After washing with PBS containing 0.01% Triton-X, the slices were incubated with 594-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 h at room temperature. .. To determine the muscle fiber type, the slices were incubated with primary antibodies against MyHC I (mouse, monoclonal, BA-F8; Developmental Studies Hybridoma Bank IA, USA), MyHC IIa (mouse, monoclonal, SC-71; Developmental Studies Hybridoma Bank), and Article Title: Non-muscle myosin IIC predominantly expressed in the slow-twitch skeletal muscles impedes age-related muscle weakness Article Snippet: After washing with PBS containing 0.01% Triton-X, the slices were incubated with 594-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 h at room temperature. .. To determine the muscle fiber type, the slices were incubated with primary antibodies against MyHC I (mouse, monoclonal, BA-F8; Developmental Studies Hybridoma Bank IA, USA), MyHC IIa (mouse, monoclonal, SC-71; Developmental Studies Hybridoma Bank), and Article Title: Displaced myonuclei are attributable to both resident myonuclear migration and stem cell fusion during mechanical loading in adult skeletal muscle Article Snippet: .. For fibre type assessments, sections were incubated in primaries for |
